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Figure 5 Loss of foxc1 does not significantly change cilia length or left–right organizer flow. (A) Acetylated α-tubulin immunostaining of left–right organizer (LRO) cilia revealed that average cilia length was not significantly altered in foxc1 mutants (foxc1a−/−, 82%; foxc1b−/−, 93%; foxc1a−/−; foxc1b−/−, 82% of WT length; p = 0.286, ANOVA; 4–11 embryos imaged per condition, all cilia per condition shown in light grey). Tracking of fluorescent bead flow in the LRO revealed unchanged counterclockwise flow between WT (B), foxc1a−/− homozygotes (B’), and foxc1a/foxc1b morphants (B’’).
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