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Figure Caption

Figure 3—figure supplement 1. MEP glia do not exit the spinal cord infoxd3mutant larvae.

(A) In situ hybridization showing wif1+ MEP glia (yellow outlined arrowheads) along the motor nerve root in a foxd3+/- control larva and wif1+ MEP glia (black arrowheads) in the spinal cord of foxd3-/- larva at 3 dpf. (B) Lateral view of a foxd3:mcherry; olig2:egfp;foxd3-/- larva at 3 dpf showing a foxd3+ MEP glia in the CNS (white arrowhead) and olig2+ OPCs in the PNS (yellow outlined arrowheads). Dashed box shows single z-plane confocal image of a HuC immunohistostaining showing olig2+/HuC+ motorneurons and a foxd3+/HuC- MEP glial cell (white arrowhead). Scale bar, (A–B) 10 µm.

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