ZFIN Image: Fontenas et al., 2021, Figure 1—figure supplement 2

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Figure Caption

Figure 1—figure supplement 2—source data 1. Fluorescence intensity over the timecourse of MEP glial development.

(A) Single z-plane confocal images of the motor exit points showing foxd3⁺/nkx2.2a⁺ MEP glia (arrowheads) at 48, 50, 55,and 72 hpf in foxd3:mcherry;nkx2.2a:nls-egfp embryos. (B) Single z-plane confocal images of the motor exit points showing foxd3⁺/olig2⁺ MEP glia (arrowheads) at 48, 50, 55 hpf and foxd3⁺/olig2^- MEP glia (arrowheads) at 72 hpf in foxd3:mcherry;olig2:egfp embryos. (C) Relative GFP fluorescence intensity of MEP glial cells. Mean ± SEM of nkx2.2a fluorescence levels at 48hpf: 1 ± 0.05; 50hpf: 0.92 ± 0.06, p=0.53; 55hpf: 0.79 ± 0.06, p=0.03; 72hpf: 0.76 ± 0.05, p=0.02. Mean ± SEM of olig2 fluorescence levels at 48hpf: 1 ± 0.04; 50hpf: 0.62 ± 0.03, p<0.0001; 55hpf: 0.47 ± 0.03, p<0.0001; 72hpf: 0.36 ± 0.02, p<0.0001. (A–B) Scale bar, 20 μm.

Acknowledgments

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