Fig. 4

Fig. 4

Evaluation of the knockdown effect by immunohistochemistry using an anti-GRK7 antibody. Double immunostaining for GRK7 (magenta) and PP1 (green) in the knockdown zebrafish larvae. a Control MO knockdown, (b) GRK7a-MO1 knockdown, (c) GRK7a-MO2 knockdown, (d) GRK7b-MO1 knockdown and (e) GRK7b-MO2 knockdown. The white dotted traces indicate the landmarks of the pineal organ. The yellow arrowheads indicate PP1 cells in the pineal organ of larval zebrafish. The scale bar represents 25 μm. The rostral (R) and caudal (C) sides are shown. f Quantitative analyses of GRK7 immunoreactivity in different GRK MO- or control MO-injected larvae. The ratios of GRK7 immunoreactivity to PP1 immunoreactivity in PP1 cells are compared. The error bars indicate the standard error. Significance scores represent P values determined by Wilcoxon rank sum test (**, P < 0.01, ***, P < 0.001, NS, nonsignificant, P > 0.05)