ZFIN Image: El Fersioui et al., 2021, Fig 4

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Figure Caption

Fig 4 Hmx1 mutant zebrafish generation via zinc-finger nuclease method.

(A) Wildtype and hmx1 mutant sequence analysis. Hmx1^mut150 mutant embryos carried a 170 bp deletion and a 21 bp insertion, while Hmx1^mut10 embryos reported a 10 bp deletion. (B,D) ExPasy translation tool depicted the disruption of the encoding sequence of the homeodomain and the downstream region composed of SD1 domains in exon2. C. Agarose gel of PCR amplified DNA products. The DNA isolated from WT, hmx1^mut10 and hmx1^mut150 embryos was amplified by PCR.

Acknowledgments

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