|
Fig. 3 Figure 3Akr1a1b Knockout in Zebrafish Caused Alterations of the Embryonic Pronephros (A) Compared with akr1a1b+/+ embryos (n = 33, mean ± SD) at 48 hpf, Δ+17 akr1a1b-/- mutants (n = 21, mean ± SD) displayed an enlarged glomerulus (encircled) and shortened tubular neck (asterisk). (B) Representative pronephros images of 48hpf old akr1a1b+/+ and Δ+17 akr1a1b-/- embryos. (C) A significant increased loss of fluorescence in Δ+17akr1a1b-/- mutants (n = 18, mean ± SD) was observed when compared with akr1a1b+/+ larvae (n = 21, mean ± SD), which indicated an altered glomerular filtration rate in akr1a1b-/- mutants. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001, p value was calculated by t test. Scale bar: 0.1 mm.