Figure 5

Figure 5

Effect of PTP1B overexpression in SH-SY5Y on PTP1B expression and neuroprotective effect against 6-OHDA damage. (a) PTP1B-overexpressed SH-SY5Y was established by the transfection of PTP1B plasmid. Western blotting for PTP1B of the control, #1 PTP1B-overexpressed SH-SY5Y, and #2 PTP1B-overexpressed SH-SY5Y groups are shown. (b) Normal SH-SY5Y cells were pretreated with 0.01, 0.1, 1, and 10 μM suramin for 1 h and then challenged with 20 μM 6-OHDA for 16 h in the control, 6-OHDA, 6-OHDA plus suramin, and suramin alone groups. Data are presented as mean ± SEM, and each value contains three replicates and six samples. ^∗Significantly different from the 6-OHDA group. (c) PTP1B-overexpressed SH-SY5Y was established by the transfection of PTP1B plasmid. Cell viability of the control; 6-OHDA; and 6-OHDA plus 100, 10, 1, and 0.1 μM suramin groups was measured. Data are presented as mean ± SEM, and each value contains three replicates and three samples. ^∗Significantly different from the control (negative control) group. ^#Significantly different from the 6-OHDA (negative control) group.