Figure 3

Figure 3

Zebrafish lpin1 morphant embryos showed defects in the skeletal muscle at 4 dpf. (A-C') Classification of the lpin1 morphants was based on the trunk phenotype (A-C) and the corresponding reduction in birefringence observed in skeletal muscles (A'-C'). Class I (A, A') represents the wild-type-like phenotype with neither defect in embryonic trunk nor reduced birefringence in the skeletal muscle. Scale bar: 200 μm. (D) The phenotype of lpin1 morphants was rescued by the co-injected mRNA of human LPIN1^wt but not LPIN1^p.I683L, LPIN1^p.R734Q or LPIN1^{p.I683L& R734Q}. (E) Quantification data are calculated by the mean intensity of 10 somites (from somite 5 to 15) for each embryo using Image J, which is normalized to that of class I (control) to obtain percentage values. n = 10 embryos for each class. *** indicates p < 0.001 by Student's t-test.