Figure 5—figure supplement 1.

Figure 5—figure supplement 1.

(A) Schematic of the emx2 genomic structure and its replacement with donor DNA using CRISPR. Red arrowhead indicates the target position for the guide RNA. The reporter eGfp and a polyA sequence in the targeting vector are flanked by left and right homologous arm (LHA and RHA) of the emx2 allele as indicated with dotted line. The targeting position for the guide RNA is not included in either the LHA or RHA. (B) Hybridization signals of emx2 transcripts in the brain (b), otic vesicles (ov), and pharyngeal arches (pa). (C) Gfp-positive regions in emx2:Gfp reporter larvae are similar to emx2 positive regions indicated by in situ hybridization.