Fig. 3

Fig. 3

Muscle endurance test for adult F1 TPM3 transgenic zebrafish. a Critical swimming speed (U_crit) was used to measure the muscle endurance using a swimming tunnel. The statistical significance was calculated in comparison with AB(WT). The orange plot is AB(WT) (n = 10), red plot is the four TG lines of TPM3(WT) (TG1, n = 8; TG2, n = 8; TG3, n = 8; TG4, n = 8), blue plot is the four TG lines of TPM3(E151A) (TG1, n = 8; TG2, n = 7; TG3, n = 8; TG4, n = 7), green plot is the four TG lines of TPM3(E151G) (TG1, n = 8; TG2, n = 7; TG3, n = 4; TG4, n = 9). b The body weight of the 13 groups. c The standard length of the 13 groups. d Principal component analysis of endurance test for adult F1 TPM3 transgenic zebrafish. The first principal component (PC) accounted for 52.3% and the second PC for 27.7% of the variance. The orange plot is AB(WT) (n = 10), red is TPM3(WT) (n = 24), blue is TPM3(E151A) (n = 22), and green is TPM3(E151G) (n = 19). Statistical significance was determined using a t-test, *0.01 < P ≤ 0.05; **0.001 < P ≤ 0.01; ***0.0001 < P ≤ 0.001; ****P ≤ 0.0001