Figure 5.

Figure 5.

Blocking Hh signaling with Cya reduces cyclinD1 expression in the hypothalamus but does not increase hypothalamic cell death. A, Sagittal section through a 6-dpf larval brain showing cyclinD1 mRNA expression in the ventricular regions of the hypothalamus (dashed-square), telencephalon (tel), and dorsal tectum (arrows). B, cyclinD1 is expression is eliminated in the LR and PR of the hypothalamus (arrowheads) following 12 h of Cya exposure but is largely unaffected in the tectum (arrows). C, Cell death in a DMSO-treated control larva as revealed by anti-phospho-Caspase3 antibody labeling. D, Two-day Cya treatment did not lead to increased cell death in the hypothalamus, although increased cell death was seen in the tectum (arrows). cb, cerebellum; tect, tectum; tel, telencephalon. Scale bars: 50 μm.