ZFIN Image: Chin et al., 2020, Figure 7.

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Figure Caption

Figure 7.

Zebrafish stag2b and rad21 cohesin mutants show increased sensitivity to Wnt signaling.

Wnt reporter Tg(7xTCF-Xla.Siam:nlsmCherry)^ia5 control embryos, Tg(7xTCF-Xla.Siam:nlsmCherry)^ia5;stag2b^nz207 and Tg(7xTCF-Xla.Siam:nlsmCherry)^ia5;rad21^nz171 cohesin-mutant embryos were treated with 0.15 M LiCl from 4 hpf to 20 hpf. (A–H) max projections of 4 (10 μm) optical sections. (A,C,E,G) TD (transmitted light detector) images merged with confocal images. (B,D,F,H) confocal images alone. (I,K,M,O) Brightfield/fluorescent and (J,N,L,P) confocal images of the same embryos in I,K,M,O. (A–D) and (I–J) Tg(7xTCF-Xla.Siam:nlsmCherry)^ia5 control embryos (WT) have low level fluorescence (Wnt reporter activity) in the midbrain that is increased following treatment. (E–H) Tg(7xTCF-Xla.Siam:nlsmCherry)^ia5; stag2b^nz207 embryos have elevated baseline levels of fluorescence (Wnt reporter activity) relative to controls (yellow arrow) with not much further increase upon LiCl treatment. (M–P) Tg(7xTCF-Xla.Siam:nlsmCherry)^ia5; rad21^nz171 cohesin-mutant embryos show enhanced Wnt reporter activity in the midbrain (green arrow) upon LiCl treatment, relative to controls. hpf, hours post-fertilization. mb, midbrain. Scale bar, 50 µm.

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Acknowledgments

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