ZFIN Image: Shi et al., 2020, Figure 6

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Figure 6

Depletion of NDUFA7 leads to cardiac hypertrophy in H9c2 cells. (A) H9c2 cells were transfected with control or NDUFA7 siRNAs for 72 hours, and expression level of NDUFA7 and GAPDH were examined. (B) H9c2 cells transfected with control or NDUFA7 siRNAs were stained for ROS using 2’,7’‐dichlorofluorescin diacetate, and then visualized under the fluorescence microscope. Scale bar, 50 µm. (C) Experiments were performed as in (B), and the ROS intensity in each group were examined by ImageJ. *P < .05 compared with control group. (D) H9c2 cells transfected with control or NDUFA7 siRNAs for 72 h, RNA was then extracted for RT‐qPCR analysis. **P < .01 compared with control group. (E) Schematic representation of the proposed mechanism. Depletion of ndufa7 triggered ROS production and subsequent calcineurin signalling activation, which further led to the expression of cardiac hypertrophy genes

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