ZFIN Image: Rajan et al., 2020, Fig 1

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Figure Caption

Fig 1 Characterization of perivascular fibroblasts in zebrafish.

(A) Lateral view of a three somite region in nkx3.1^NTR-mCherry; kdrl:EGFP embryos at 48 hpf. Many mCherry⁺ perivascular fibroblasts (red, arrowheads) were closely associated with intersegmental vessels (ISVs) labeled by the endothelial marker kdrl:EGFP (green). n = 11 embryos. (B) Co-expression of nkx3.1^NTR-mCherry and col1a2:GFP in perivascular fibroblasts (arrowheads) at 52 hpf. The ISV is indicated by dashed lines. Graph showing proportion of perivascular fibroblasts labeled by col1a2:GFP is shown in S1A Fig. n = 17 embryos. (C) Fluorescent mRNA in situ hybridization showing expression of fibrillar collagens col1a2 (green, left) and col5a1 (green, right) in perivascular fibroblasts (arrowheads) along ISVs marked by kdrl:EGFP (red) at 48 hpf. n = 15 embryos per staining. Scale bars: (A,C) 50 μm; (B) 25 μm.

Figure Data

Expression / Labeling details

Acknowledgments

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