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Fig. 3 Regulation of shtn‐1 expression is important for retinal pigmented epithelium development, but not retinal lamination. A‐D, Wholemount lateral images of the eye at 72 hpf following shtn‐1 and PlxnA2 expression manipulation. A'‐D", H&E stained transverse sections of the eyes and forebrain at 72 hpf. (A"‐D") zoom of right eye as seen in A'‐D'. Yellow asterisks indicate areas of missing RPE. E, Schematic of % intact RPE measurement. F, Quantification of percent intact retinal pigmented epithelium (RPE) as seen in A"‐D" (P‐values were calculated using one‐way ANOVA, Tukey's multiple comparisons posthoc test). Scale bars A‐D, 200 μm, A"‐D", 50 μm. UIC, uninjected control