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Fig. 2 PlxnA2 negatively regulates shtn‐1 expression to influence optic vesicle development. A‐D, Dorsal view at 18 hpf of shtn‐1 In situ hybridization following PlxnA2 and Shtn‐1 expression manipulation. E‐H, Dorsal confocal images of rx3:GFP embryos at 18 hours following Shtn‐1 and PlxnA2 expression manipulation. I, Quantification of eye vesicle migration (P‐values were calculated using One‐way ANOVA with multiple comparisons). J,K, Expression of midline shha at 18 hpf in control and shtn‐1 overexpression embryos. Scale bars, 100 μm. A, anterior; P, posterior, UIC, uninjected control
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