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Figure 1—figure supplement 2.

ID: ZDB-IMAGE-201021-4
Source: Figures for Helker et al., 2020

Image

Figure Caption

Figure 1—figure supplement 2.

Generation of the Tg^BAC(apln:EGFP) and Tg(aplnrb:aplnrb-TagRFP-sfGFP) reporter lines.

BAC engineering was used to generate the apln:EGFP and aplnrb:aplnrb-TagRFP-sfGFP constructs. The clone RP71-2G21 was engineered to replace the ATG of apln with an EGFP cassette. The clone CH211-102K8 was engineered to replace the stop codon of aplnrb with a TagRFP-sfGFP cassette. To enhance the frequency of insertion into the genome, tol2 arms were added to the backbone of the BAC.

Acknowledgments

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Figure 1—figure supplement 2.

Figure 1—figure supplement 2.

Generation of the TgBAC(apln:EGFP) and Tg(aplnrb:aplnrb-TagRFP-sfGFP) reporter lines.

Generation of the Tg^BAC(apln:EGFP) and Tg(aplnrb:aplnrb-TagRFP-sfGFP) reporter lines.