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Fig. 2
Schematic of the differentiation protocol timeline. Maintenance Medium (MM) = iPSC medium (StemFlex with 1× penicillin/streptomycin), NCC differentiation medium = DMEM‐F12, 10% fetal bovine serum, 1 mM sodium pyruvate, 1 mM penicillin/streptomycin, 1 mM nonessential amino acids, 110 μM 2‐mercaptoethanol, 10 ng/ml epidermal growth factor. Images of iPSC and iPSC‐derived NCC at Days 0, 14, and passage 4 following differentiation. Scale bars: 400 μm (Day 0), 200 μm (Day 14, passage 4).
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Full text @ EMBO Mol. Med.