ZFIN Image: Pini et al., 2020, Fig. EV1

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Figure Caption

Fig. EV1 Induced pluripotent stem cells (<styled-content toggle='no' style='fixed-case'>iPSC</styled-content>) derivation and <styled-content toggle='no' style='fixed-case'>EB</styled-content> generation

A

Schematic representation of the strategy used to generate iPSC. Blood samples from an unrelated normal individual, unaffected father (subject 1), and two of the affected children (subjects 5 and 6) were processed. Isolated PBMC were infected with Sendai virus, and individual clones were picked 21 days after the infection. Following expansion until passage 10, iPSC were characterized and embryoid bodies were formed by suspension culture for 14 days.

B

The reprogramming process of the PBMC showed that all cells underwent similar morphological changes leading to the formation of iPSC clones by day 21. These clones still displayed embryonic stem cells morphology at passage 10, indicating that the cells are able to self‐renew. All iPSC clones were able to form EBs. One clone of each subject is represented. Scale bar is 400 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.