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Figure 2

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ZDB-IMAGE-201003-31
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Figures for Converse et al., 2020
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Figure 2

Characterization of zip9-mutant phenotype. (A) Percent of viable eggs ovulated by zip9-/- and zip9+/+ females 2 h post fertilization (hpf); n = 13–14 clutches. (B) Representative image of zip9+/+ and zip9-/- embryos at 4 hpf and 1 and 3 days post fertilization (dpf). Scale bars: 500 µm. Arrows donate the yolk sac in WT and mutants (3 dpf) and asterisks donate edema in zip9-/- larvae (3 dpf). (C) Chorion diameter of eggs spawned by zip9-/- and zip9+/+ females; n = 40. (D) Yolk volume of 3 dpf zip9-/- and zip9+/+ larvae; n = 16–18. (E) Length of zip9-/- and zip9+/+ larvae without exogenous feeding between 3–10 dpf; n = 10–21. (F) Incidence of pericardial/yolk sac edema in 6 dpf zip9-/- and zip9+/+ larvae; n = 5 clutches. All data represents means ± SEM. Significance was determined by Welch's t-test (*, P < 0.05; **, P < 0.001; ***, P < 0.0001).

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