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Fig. 4 MO induced loss of Mci function does not have a major effect on MCC formation in the zebrafish kidney tubules. (A) Compared to wild-type embryos (uninjected), embryos injected with a translation-blocking MO against mci displayed reduced number of MCC bundles in the kidney tubules at 48 hpf. In the MCC bundles that still persisted in the morphant kidneys, basal body duplication appeared normal (the γ-tub channel in the quartet of small panels; brackets delineate duplicated basal bodies in individual MCCs). Scale bars = 10 μm. (B) Counting of the number of MCC bundles per kidney tubule showed that while wild-type 48 hpf embryos harbored close to 20 MCC bundles per duct on average, approximately 10 bundles were present, on average, in morphants. The graph was plotted using the average of averages from 3 independent experiments (n = 10–15 for each experiment). Error bars represent SD. Statistical analysis is the result of two-tailed, unpaired t-test (p = 0.0003, ∗∗∗). (C, D) Analysis of foxj1b::gfp expression revealed that at 30 hpf, mci morphants displayed fewer numbers of foxj1b-positive (foxj1b+) cells per tubule, compared to wild-type controls (C). Scale bar = 10 μm. Counting of the number of foxj1b+ cells per kidney tubule (D) revealed that while wild-type ducts harbored approximately 20 foxj1b+ cells per duct at 30 hpf, the morphant embryos had about 15 cells on average. The graph was plotted using the average of averages from 3 independent experiments (n = 10–15 for each experiment). Error bars represent SD. Statistical analysis is the result of two-tailed, unpaired t-test (p = 0.0022, ∗∗).
Reprinted from Developmental Biology, 465(2), Zhou, F., Rayamajhi, D., Ravi, V., Narasimhan, V., Chong, Y.L., Lu, H., Venkatesh, B., Roy, S., Conservation as well as divergence in Mcidas function underlies the differentiation of multiciliated cells in vertebrates, 168-177, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.