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Figure 3
Co-immunoprecipitation analysis for the interaction of Udu with Bap1, Dnmt1, Thoc1, and Cry3a. Flag-tagged control or Flag-tagged Udu-expressing constructs were co-transfected with different Myc-tagged candidate Udu-interacting proteins, including Bap1, Dnmt1, Thoc1, and Cry3a. The cell lysate was incubated with anti-FLAG M2 magnetic beads, then subjected to immunoblot with anti-MYC or anti-FLAG antibody. Arrowheads indicate the expected size of full-length Udu. Lanes 1 to 9 were cropped from the same blots, while lanes 10–12 were from the other ones. To observe the expression of full-length Udu, photographs using a longer exposure time of anti-FLAG immunoblots were taken. The original un-cropped immunoblots were shown in Supplementary Fig. S7a and photographs with shorter exposure time for anti-FLAG immunoblots were also included in Supplementary Fig. S7b as supporting information.