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Figure 6.

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ZDB-IMAGE-200829-70
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Figures for Liu et al., 2020
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Figure 6.

Defective organization of F-actin in tmem216 knockout photoreceptors. Cryosections were immunostained with 4D2 (red). Some sections were stained with Phalloidin-RITC for F-actin (red). (A, C) 4D2 staining of wildtype and tmem216snyΔ175 homozygous retina. 4D2 labels rod outer segments in the wildtype zebrafish (arrow in A); however, its reactivity was frequently mislocalized to the rod cell body in the knockouts (arrowheads in C). (B, D) Phalloidin staining in wildtype and tmem216snyΔ175 homozygous retina. Vertically oriented F-actin in the wildtype (arrows in B) were disrupted in tmem216snyΔ175 homozygous retina (arrowheads in D). Scale bar in H: 6 µm.

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