ZFIN Image: Liu et al., 2020, FIGURE 1

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Figure Caption

FIGURE 1

Targeted deletion of miR-430 in zebrafish. (A) Genetic deletion of zebrafish miR-430 cluster using TALENs. A 79.8 kb genomic fragment containing the zebrafish miR-430 was deleted using two pairs of TALENs. The TALEN binding sites were shown in colors and the inserted nucleotides were shown in lower case letters. MiR-430 isoforms were indicated by colored short lines. (B)In situ hybridization detection of pri-mir-430 expression in the WT and miR-430^–/– mutant embryos at 4 hpf. (C) Expression profiles of mature miR-430 isoforms in the WT and miR-430^–/– mutant embryos (12 hpf) by small RNA deep sequencing. The numbers of normalized reads were indicated. TPM, transcripts per kilobase million.

Acknowledgments

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