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Fig. S4
Optimization of light-inducible, neuron-specific gene expression using the GAVPO system. (A-B) Tg(elavl3:GAVPO; UAS:NTR-mCherry) embryos were irradiated globally for varying durations, starting at 48 hpf and using a (A) blue LED lamp or (B) white LED lamp. The embryos were imaged at 56 hpf, and representative brightfield and epifluorescence micrographs are shown. (C) Quantification of mCherry fluorescence in Tg(elavl3:GAVPO;UAS:NTR-mCherry) embryos irradiated as described in A-B. Embryo orientations:lateral view and anterior left. Scale bars: 500 μm.
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