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Fig. 1 S1
Cardiomyocytes change morphology and exhibit cell-cell junction rearrangements when entering the trabecular layer.
(a) Schematic of the transplantation experiment. (b) Circularity analysis of CMs in the compact and trabecular layers (n = 7–9 CMs from 3 to 5 ventricles). (c–c”) 3D images of chimeric hearts; Y-Z plane images (d–d”) along white dashed line (c”). A host-derived CM entering the trabecular layer exhibits membrane protrusions (c, d, c’, d’; arrows). (e) Schematic of the transplantation experiment. (f–f”, h–h”) 3D images of chimeric hearts; Y-Z plane images (g–g”, i–i”). A CM entering the trabecular layer exhibits N-cadherin localization in protruding membranes (g, g’; arrows), while a CM remaining in the compact layer exhibits lateral localization of N-cadherin (i, i’; arrowheads). L, cardiac lumen. Error bars, s.e.m.; **p<0.001 by two-tailed unpaired t-test. Scale bars, 20 μm.