ZFIN Image: Takamiya et al., 2020, Fig 5

Image

Figure Caption

Fig 5 Endothelial cells emerge in a concentric ring pattern in the nascent cornea.

(A-C”) The Tg(corneaEndo:GFP) line shows the first corneal endothelium cells (green) as a patch of cells over the lens (arrows in A, 60–72 hpf) that later spread across the entire inner surface of the cornea (B: 96 hpf; C:120 hpf). (C’-C”‘) Transverse (C’) and horizontal (C”‘) optical sections along the stippled lines in C, merged onto pictures from reflection imaging (grey channel) which highlight the corneal epithelium (Ep; note: the strong lateral signals are iridophores). (C”) Magnified view of the central cornea region indicated by a rectangle in C’. En: corneal endothelium; Ln: lens; Ir: iris; Scale bars: 50 μm (A-C, C’ and C”‘); 10 μm (C”). (D) The AS development is classified into five phases. Phase 1: 2°NC cell cluster formation (18–22 hpf); Phase 2: 2°NC cluster dissolves and migrate into the eye (22–28 hpf); Phase 3: NC cells differentiate into ocular mesenchymal cells (28–60 hpf); Phase 4: First corneal endothelial cells emerge over the lens (60–72 hpf); Phase 5: Corneal endothelium fully covers the eye (>72 hpf).

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.