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Fig. 7
Inhibition of Cx43-GJIC rescues cx43lh10 phenotypes. (A) Gap27 injection rescues regenerate length in cx43lh10 fins. Fins were amputated at the 50% level. The amputation plane is indicated (black line). Black arrows indicate the distance from the amputation plane to the distal tip of the third fin ray. Bar graph shows reduced regenerate length in cx43lh10 fins injected with Gap27 compared with cx43lh10 fins injected with Scrm-Gap27 (Student's t-test, two tailed and unpaired, *P<0.05). n=14; three independent replicates. (B) Gap27 injection rescues segment length in cx43lh10 fins. Representative images are shown. Arrows and arrowheads point to the first two joints following the amputation plane (white line). The inset shows a higher magnification of the segments. Bar graph shows reduced segment length in cx43lh10 fins injected with Gap27 compared with cx43lh10 fins injected with Scrm-Gap27. Student's t-test, two-tailed and unpaired, *P<0.05 (n=14, three independent replicates). Data are mean±s.e.m. Scale bar: 50 µm.