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Fig. 6
The cx43lh10 allele exhibits Cx43 overexpression phenotypes. (A) Schematic of Cx43 protein showing the location of the carboxy-tail deletion. N, amino terminus; C, carboxy terminus; EL1/EL2, extracellular loop1/2; CL, cytoplasmic loop. (B) Regenerating fins in cx43lh10mutants exhibit longer regenerate length compared with wild type (WT). All fins were amputated at the 50% level. The amputation plane is indicated (black line). Black arrows indicate the distance from the amputation plane to the distal tip of the third fin ray. Bar graph shows a significant reduction in regenerate length between cx43lh10 and wild type (Student's t-test, two tailed and unpaired, *P<0.05). n=22; three independent replicates. (C) Regenerating fins in cx43lh10 mutants exhibit longer fin ray segments compared with wild type. Arrows and arrowheads point to the first two joints following the amputation plane (white line). The inset shows a higher magnification of the segments. Bar graph reveals a significant reduction in segment length between cx43lh10 and wild type (Student's t-test, two-tailed and unpaired, *P<0.05). n=23; three independent replicates. (D) Regenerating fins in cx43lh10 mutants exhibit reduced evx1 expression compared with wild type. + indicates fin rays positive for evx1; − indicates fin rays negative for evx1. Bar graph shows the percentage of evx1+ fin rays is reduced in cx43lh10 compared with wild type. Student's t-test, two tailed and unpaired, *P<0.05. (n=10; three independent replicates). Data are mean±s.e.m.