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Fig. 3
Cx43-GJIC influences changes in gene expression. (A) Proposed pathway for changes in gene expression influenced by Cx43-GJIC. (B) Gap27 injections lead to reduced smpexpression compared with Scrm-Gap27 injections (n=15). Asterisks indicate identification of stained fin rays. (C) Gap27 injections lead to increased frequency of evx1 expression compared with Scrm-Gap27 injections (n=10, three independent replicates). + indicates fin rays positive for evx1; − indicates fin rays negative for evx1. (D) Bar graph showing increased percentage of evx1+ fin rays with Gap27 injections compared with Scrm-Gap27 injections (Student's t-test, two tailed and unpaired, *P<0.05). n=16; three independent replicates. Data are mean±s.e.m. Scale bar: 50 µm.