|
Fig. s6 Expression of the different components of the Wnt and PCP pathways, and hair cell orientation in the Occipital and Dorsal lateral lines. a. Low magnification picture of wnt11 (wnt11f1) mRNA expression in the trunk of a 50hpf Wild type fish. wnt11 (wnt11f1) is expressed along the myoseptum. b. t-SNE plots and violin plots showing expression of fzd7a, gpc4 and scrib in a 5dpf homeostatic neuromast. The Wilcox p-value for gpc4 equals 6.4 x 10^-4 in support cells and mantle cells versus hair cells, while in fzd7a and scrib the expression pattern was too sparse to generate meaningful statistics. c. Hair cell orientation of neuromasts in the Occipital and Dorsal lateral lines of Wild type and MZwnt11 (wnt11f1) mutants. Neuromasts that show a hair cell phenotype in MZwnt11 (wnt11f1) mutants are labeled as concentric. D1=Dorsal 1. D2= Dorsal 2 (belonging to the dorsal lateral line). Scale bar in a equals 50µm; c equals 5µm.