ZFIN Image: Blume et al., 2020, Fig. 6

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Figure Caption

Fig. 6 Pharmacological inhibition of P2RY12 signaling results in delayed clearance of apoptotic cells in the developing retina. Mpeg1 :mCherry embryos were pretreated with PSB?0739 for 1 hour, then imaged with continual immersion in PSB?0739. Acridine orange (AO, green) was used to label apoptotic cells in vivo. A. Selected z planes and timeframes are shown for imaging in the presence of PSB?0739. Isolated AO+ cells (arrows) are detected, which are ignored by microglia. Time stamp in upper right (hour:minute) begins at appearance of the first AO+ cell (arrow, left panel). Scale bar in, A, applies to all panels. B, Box plots represent the total number of AO+ or PtdSer+ cells detected during 8 hours of imaging in control or PSB?0739 treated retinas (n = 6?7 eyes from 6 embryos per group). Individual measurements are shown in circles and are overlaid on the box plots. A one?way ANOVA (P = .00016) was performed, followed by Tukey's HSD post hoc test. P values shown for pairwise comparison with P ?

Acknowledgments

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