ZFIN Image: Xia et al., 2020, Fig. S2

Image

Figure Caption

Fig. S2

Verification of the ube3d knockout efficiency in zebrafish.

(A)RT-PCR was used to measure ube3d expression in 24 hpf wild-type zebrafish、e2 MO ube3d-knockdown zebrafish and blank control group

(under normal circumstances, the length of ube3d is 524bp; due to the deletion length of exon 2 of ube3d, the length of e2 MO ube3d-knockdown zebrafish change to be 230bp). The following figure at right shows the bright field morphology of wild-type 24hpf and ATG-MO ube3d-knockdown

zebrafish;the following figure on the left shows the expression of the ATG-

MO target sequence (green fluorescence table) in the wild-type 24hpf and the ATG-MO zebrafish.

Acknowledgments

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