Fig. 4

Fig. 4 Mitochondrial membrane potential partly restored after mcu deletion. The graph represents ratio of JC-1 dye emission spectrum (590 nm to 530 nm). wt mitochondria showed a slight uptake of JC-1, which turned into leakage after ∼18 min after the start of the experiment, when mitochondria began to lose membrane integrity in an in vitro environment. pink1^−/− mitochondria did not show uptake, instead we observed JC-1 leakage starting at ∼6 min after experiment initialization. Both variants started and ended at similar levels. Results are mean with s.e.m. [wt had two, pink1^−/− had two and pink1; mcu)^−/− had one independent replication(s)] gathered during two independent experiments. Every replication was done on 200 larvae per variant.