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Fig. 8 Myh11-positive endocardial cells and thrombocytes retain their double identity. (A-B′) In situ hybridisation for itga2b combined with immunohistochemistry for Citrine and Myh11. Arrowheads indicate Myh11-positive itga2b-positive thrombocytes present in the wild-type wound that are largely missing in the runx1 mutant wound at 7 dpci. (C-D′) In situ hybridisation for itga2b combined with immunohistochemistry for Myh11 with nuclear Dapi staining. Both the endocardium (inset) and thrombocytes (arrowheads) still express Myh11 in the wild-type wound at 14 dpci, while being absent in the mutant wound. v, ventricle; w, wound. Scale bars: 100 μm.