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Figure 5-figure supplement 4 Intracellular calcium measurement in C2C12 myotubes.

Ratiometric fura-2 imaging with 10 mM caffeine induction after treatment with D) SB203580, (E) SB202190, and F) p38 MAPK siRNAs. Panels on the left show the peak change in calcium concentration from [Ca2+]i within 30 s of adding caffeine without normalization (i.e. Δ ratio), while plots on the right show [Ca2+]i followed by changes in fura-2 340/380 nm ratio after addition of caffeine.

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