Figure 2-figure supplement 1

Figure 2-figure supplement 1

(A) The ‘thrashing assay’ is performed by counting the waveforms propagated by individual C. elegans in one minute (Maryon et al., 1996). Consistent with previous reports (Maryon et al., 1996; Maryon et al., 1998), we found that unc-68 mutants thrash at a significantly lower rate than N2 (wild type) in two independent assays (n = 10 per genotype; Student’s T-test: ***p<0.001). (B) In both liquid and solid media, the DHPR inhibitor nemadipine-A (Kwok et al., 2006) induces concentration-dependent developmental growth arrest in unc-68(r1161) (strain TR2170) and unc-68(r1162) (strain TR2171) mutants. L1 larvae were plated in chemical at Day 0 (n = 20/well liquid 96-well plate; n = 50/well solid 24-well plate) and developmental stage was measured at Day 6 (liquid) and Day 5 (solid). Abbreviations: og = overgrown with larvae/eggs and food source depleted; tnc = larvae and eggs were too numerous to count, but food source not depleted; ne: no eggs have been laid (this phenotype is consistent with inhibition of egg-laying defective protein EGL-19, a DHPR, by nemadipine-A).