Co-staining of cerebellum with Casq and neuron-specific markers. (A–C) Epi-fluoresence signal obtained by double immunofluorescence with CC antibody (green) and anti-parvalbunin antibodies (red) in the cerebellum area. (C) A merge of the two images. Arrowheads indicate the axons of Purkinje cells. (D–F) Epi-fluoresence images obtained by double-staining with MC antibody (green) and anti-parvalbunin antibodies (red). (D) A merge of the two images. (G–I) Confocal images obtained by double-staining with MC antibody (green) and anti-NeuroD1 antibody (red). (J–L) Higher magnification of granule cells in the central area of the cerebellum double-labeled with MC (green) and anti-NeuroD1 antibody (red). Bar: 25 μm.
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