Fig 1

Fig 1

(A) Flowchart of FACS and RNA-seq analysis of the different types of cells in the trunk region from Tg(kdrl:mCherry/runx1:en-GFP) transgenic zebrafish embryos at about 28 hpf. (B) GO analysis of the sequencing data shows that up-regulated transcripts in runx1⁺ HE are enriched in the endosome and endocytic trafficking related terms. (C) RPKM values of Rab5 family genes in EC and HE. (D) Expression pattern of rab5c mRNA examined by WISH and double FISH. Scale bar, 200 μm. The P values in this figure were calculated by Student t test. The underlying data in this figure can be found in S1 Data. CA, caudal vein; DA, dorsal aorta; EC, endothelial cell; FACS, fluorescence-activated cell sorting; FISH, fluorescence in situ hybridization; GO, gene ontology; HE, hemogenic endothelium; hpf, hours post fertilization; RNA-seq, RNA sequencing; RPKM, reads per kilobase of exon model per million mapped reads; WISH, whole-mount in situ hybridization.