ZFIN Image: Carter et al., 2020, FIGURE 7

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Figure Caption

FIGURE 7

Rab28 transgenic zebrafish have normal retinal ultrastructure and normal outer segment shedding. (A) Representative TEM of 7 mpf eGFP-Rab28 transgenic zebrafish. Low magnification images show rows of several cone photoreceptors, while high magnification images show examples of OS tips. Low magnification scale bars 5 μm, high magnification scale bars 500 nm. (B–D) Confocal z-projections of 1 mpf retinas of zebrafish expressing eGFP-Rab28 (WT, Q72L, or T26N variants). Phagosomes, labeled with eGFP, are indicated by white arrows. Samples were collected 4 h after lights on. Scale bars 10 μm. (E) Scatter plots showing normalized phagosome density in the retinas eGFP-Rab28 transgenic zebrafish. Data are derived from cryosections in which eGFP was used to identify phagosomes. p-Value derived from one-way ANOVA. Error bars show SEM. Data are from 17, 17, and 13 retinal z-projections from at least three individuals for WT, Q72L, and T26N variants, respectively.

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Acknowledgments

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