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Figure 1
An in vivo, light-dependent, oligomerization switch for the formation or dissolution of Aβ aggregates in the fruit fly and nematode.
(a) A schematic of the strategy and a relative size comparison of the three components of UAS-Aβ-CRY2-mCh. (b) Expression of AD-Gal4/UAS-Aβ-CRY2-mCh in a Drosophila embryo. (c) Mean intensity of aggregates in the same AD-Gal4/UAS-Aβ-CRY2-mChDrosophilaembryo. (d and e) Expression of Elav-Gal4/UAS-Aβ-CRY2-mCh in a Drosophila larva and embryo. (f) Expression of hsp-16–2 p-Aβ-CRY2-mCh in heat-shocked C. elegans with myo-2-gfp marker, that marks its pharyngeal pump and serves as an indicator for positive microinjection. (g and h) Mean intensity of aggregates in the hsp-16–2 p-Aβ-CRY2-mCh in C.elegans under 20x and 63x magnification of the confocal microscope; 63x image was processed using the Zeiss Airyscan.