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Fig. 4 Disrupting all the estrogen receptors in dmrt1 mutants. (A) Sex ratios at 75 dpf in different mutation combinations of five genes (dmrt1, cyp19a1a, esr1, esr2a and esr2b). The data were pooled from fish of five independent breedings to enlarge the sample size. (B) Representative histological sections corresponding to different gonadal types, including the ovaries in heterozygous control, quadruple knockout (dmrt1−/−; esr1−/−;esr2a−/−;esr2b−/−) and quintuple knockout (dmrt1−/−;cyp19a1a−/−;esr1−/−;esr2a−/−; esr2b−/−) at 75 dpf (n=9-43 for each genotype). Arrows indicate atresic/degenerating follicles. PV, pre-vitellogenic; EV, early vitellogenic.