Fig. 4

Fig. 4 pnina is allelic to plod3. (A,B) Transverse histological sections through eyes of 3 dpf normal sibling (A) and plod3^tv205/tv205 (B) embryos (n = 10 embryos from each genotype). (C,D) Live 3 dpf embryos from a cross between pnina^vu222/+ and plod3^tv205/+ fish. From over 200 embryos, approximately 75% of embryos appeared normal (top embryo) and 25% showed a plod3 mutant-like phenotype (bottom embryo). C is lateral view, D, dorsal view (compare to Fig. 1A,B’,C’). (E) Relative plod3 expression as measured by RT-qPCR in plod3^vu222/vu222 mutant and normal embryos. Blue lines show mean expression level (relative quantity, RQ) and bars represent highest and lowest expression levels based on the standard error of the delta Ct’s. (F,G) Transverse sections through eyes of 30 hpf (F) and 48 hpf (G) wild-type embryos labeled for plod3 expression by in situ RNA hybridization (n = 10 eyes for each time point). Arrows in E point at the ALE and in F at CMZs. Scale bars are 50 μm (A,B), 1 mm (C), 100 μm (D), 20 μm (F,G).