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Fig. 5 Development of lens cellular mass phenotype. (A) Live imaging of a lens of a ~72 hpf plod3vu222/vu222 embryo carrying a mem-EGFP transgene (n = 5 embryos). Dorsal view, anterior to the top. ALE cells forming a multi-layered cell mass are seen next to the lens. (B–E) Transverse histological sections from eyes of plod3vu222/vu222 embryos at 75 hpf (B), 4 dpf (C,D) and 5 dpf (E) (n = 10 eyes for each time point). Red lines encircle major parts of cellular masses in B-D and a region of the mass forming a rounded pattern in E. L, original lenses. Scale bars are 20 μm.
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Reprinted from Developmental Biology, 458(2), Taler, K., Weiss, O., Rotem, S., Rubinstein, A.M., Seritrakul, P., Gross, J.M., Inbal, A., Lysyl hydroxylase 3 is required for normal lens capsule formation and maintenance of lens epithelium integrity and fate, 177-188, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.