ZFIN Image: He et al., 2020, Figure 6

Image

Figure Caption

Figure 6 <italic><styled-content toggle='no' style='fixed-case'>FST</styled-content></italic> targeting by <styled-content toggle='no' style='fixed-case'>CRISPR</styled-content>/Cas9 or antisense oligo significantly reduced leukemia cell growth <italic>in vitro</italic> and <italic>in vivo</italic>

A, B

The morphology and clonogenicity of MOLM‐13 after FST knockout by CRISPR/Cas9 in vitro. Scale bar = 10 μm.

C, D

The engraftment of MOLM‐13 after FST knockout was detected by flow cytometry of human CD45‐ and mouse CD45.1‐positive cells in recipient mouse BM aspiration at week 2 post‐transplantation.

E

The effect of FST knockout on the survival of NSG mice engrafted with MOLM‐13 cells. Cas9: Cas9 only (10 mice); sgRNA#3: Cas9 + sgRNA#3 (10 mice); sgRNA#4: Cas9 + sgRNA#4 (8 mice).

F

The knockdown efficiency of different FST‐specific antisense oligos (ASOs) in MOLM‐13 cells was detected by RT–qPCR after 3 days of treatment in vitro. The knockdown and RT–qPCR experiments were performed in triplicates.

G

MOLM‐13 cell growth was measured after 3 days of treatment of FST‐ASO in vitro. The ASO treatment experiments were performed in triplicates.

H

Intraperitoneal injection of FST‐ASO (10 mg/kg weekly, 6 mice) significantly prolonged the survival of MOLM‐13‐engrafted NSG mice. The random sequence was used for negative control (Neg‐ASO, 6 mice).

Data information: In (B, D, F, and G), data were presented as mean ± SEM. *P < 0.05 and **P < 0.01 (Student's t‐test). In (E and H), survival curves were analyzed by log‐rank test. *P < 0.05.

Acknowledgments

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