Average distance moved before (Figure 1A) and during startle stimuli (Figure 1B) in wild type and slit3sa1569 mutant five-day-old zebrafish larvae.
(A) Basal distance moved as function of amisulpride dose and slit3 genotype. The effect of dose and genotype and their interaction was tested in a linear mixed model. Timepoint, well where the fish were placed and plate were also included as fixed factors and the fish ID as random factor. (B) Distance moved during taps as function of amisulpride dose and slit3 genotype. Dose and genotype and their interaction were examined in a linear mixed model including stimulus number, well, plate used and distance moved before stimuli as fixed factors and Fish ID as random factor. Zebrafish larvae did not differ in the distance travelled before or during startle stimuli as a function of amisulpride dose nor genotype (p>0.05). Bars represent estimated marginal means ± SEM (n = 42–48 fish per group).
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