Fig. 2

Fig. 2

a Live cell imaging of support cells by Tg(tnks1bp1:GFP) and hair cells by Tg(atoh1a:dTomato) in lateral line neuromasts of the gemin5^hg81 control and mutant embryos at 2 days post hair cell ablation. Scale bar, 10 µm. b Live cell labeling of neuromast cells by Tg(cldnb:EGFP) in the lateral line of the gemin5^hg81 control and mutant embryos at 2 days post hair cell ablation. Dotted white circle demarcates the periphery of the neuromast. Scale bar, 10 µm. c Alkaline phosphatase staining of lateral line neuromasts in the gemin5^hg81 control and mutant embryos at 2 days post hair cell ablation. Arrows point to the neuromasts, which are significantly smaller in the mutants when compared to those in the control larvae. Scale bar, 50 µm. d Confocal images of lateral line neuromasts in the gemin5^hg81 control and mutant embryos at 2 days post hair cell ablation, stained with anti-hair cell antibodies (green color) and DAPI (blue color). Representative images are shown. Scale bar, 10 µm. e Quantification of neuromast cells. Error bars in the graphs represent mean ± s.e.m. There is a significant reduction in the number of neuromast cells (****P < 0.0001). The numbers are presented as percentage because they were obtained from quantification of still confocal images. Each data point was generated from ~10 embryos.