Fig. 1
Fig. 1 Comparison of the expression patterns of pou5f3 and neurogenesis genes in the neural plate. A. The expression of pou5f3 and various neurogenesis genes, including proneural genes (neurog1, ebf2), a her gene (her4.1), and Notch-related genes (deltaA, notch1a), in the neural plate was compared at the bud stage using WISH. Dorsal views with anterior to the top. Top row, dorsal views of whole embryos; bottom row, enlarged views of the areas marked with broken lines in the top row. B. The expression of pou5f3 (red) and neurogenesis genes (brown) in the midbrain-hindbrain region of the neural plate was compared by two-color in situ hybridization at the bud stage. Top row, bright-field views; middle row, epifluorescence views; bottom row, merged views. pm, primary motor neuron progenitor; rb, primary sensory neuron progenitors (Rohon-Beard neurons); r1/2, medial and lateral neuron progenitors in r1/2; r4, medial and lateral neuron progenitors in r4; tg, trigeminal ganglion; vcc, ventrocaudal cluster; vrc, ventrorostral cluster. Scale bar, 100 μm.
Reprinted from Developmental Biology, 457(1), Inomata, C., Yuikawa, T., Nakayama-Sadakiyo, Y., Kobayashi, K., Ikeda, M., Chiba, M., Konishi, C., Ishioka, A., Tsuda, S., Yamasu, K., Involvement of an Oct4-related PouV gene, pou5f3/pou2, in neurogenesis in the early neural plate of zebrafish embryos, 30-42, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.