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Fig. S13
Specific detection of Reln protein by immunostaining. WT (A, n= 4) and relnΔ7/Δ7 (B, n= 3) larvae at 5-dpf were stained with anti-Reln (magenta) antibody. Dorsal views with anterior to the left. Typical images are shown. Note that Reln signals in and near the granule cell axons were severely decreased in the reln mutants. In some reln mutant larvae, nonspecific signals were also seen bilaterally in the hindbrain (marked by arrowheads). Scale bars: 100 μm in A.
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Reprinted from Developmental Biology, 455(2), Nimura, T., Itoh, T., Hagio, H., Hayashi, T., Di Donato, V., Takeuchi, M., Itoh, T., Inoguchi, F., Sato, Y., Yamamoto, N., Katsuyama, Y., Del Bene, F., Shimizu, T., Hibi, M., Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish, 393-408, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.