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Fig. 5
Chronic inflammation and high-glucose exposure leads to α-cell expansion. (A) Confocal images (maximum projections) of islets from WT and Tg(ins:il1b) larvae at 5 dpf following incubation in either E3 or 130 mM glucose from 3 to 5 dpf. Immunostainings against insulin (green) and glucagon (magenta) mark the β-cells and the α-cells, respectively. (B) Quantification of the number of β-cells in WT and Tg(ins:il1b) larvae. Tg(ins:il1b) larvae exhibit an increase in β-cells in both E3 and glucose. Two-way ANOVA with Sidak's multiple-comparison test; *P≤0.05, **P≤0.01. Mean±s.d. (C) Quantification of the number of α-cells in WT and Tg(ins:il1b) larvae. The number of α-cells did not differ significantly (P<0.5) between WT and Tg(ins:il1b) in E3. However, there were significantly more α-cells in Tg(ins:il1b) larvae compared to WT in the glucose-treated group. Two-way ANOVA with Sidak's multiple-comparison test; *P≤0.05, **P≤0.01. Scale bars: 20 μm.
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Full text @ Dis. Model. Mech.